Skip Navigation

This Article
Right arrow Print PDF (5566K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (25)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lindquester, G. J.
Right arrow Articles by Devlin, R. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lindquester, G. J.
Right arrow Articles by Devlin, R. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 5 2099-2118
© 1989

MOLECULAR BIOLOGY

Avian tropomyosin gene expression

Gary J. Lindquester+,*, James E. Flach§, Donald E. Fleenor{varphi}, Kathryn H. Hickman and Robert B. Devlin{circ}

Emory University, Biology Department Atlanta, GA 30322, USA

*To whom correspondence should be addressed

Received September 16, 1988. Revised January 31, 1989. Accepted January 31, 1989.

Sequence analysis of overlapping fragments from a quail genomic library has revealed a tropomyosin gene consisting of 13 exons spaced over about 18 kilobase pairs of DNA. Skeletal muscle and smooth muscle transcripts share the same 5' untranslated sequence and may initiate from the same promoter. However, the regions encoding amino acids 39–80 and 258–284 are specific to each muscle type. The two sets of exons encoding these regions undergo mutually exclusive alternative splicing in a tissue-specific manner as determined by Northern blots and S1-nuclease protection. Similarly, the 3' ends of the transcripts are different in skeletal muscle and smooth muscle, and each contains two polyadenylation signals which appear to be utilized in vivo The avian alpha-tropomyosin gene is not expressed in cardiac muscle. The sequence of the gene shows great homology with other muscle-specific tropomyosins and includes a region homologous to the amino terminus of nonmuscie tropomyosins.

+Present address: Rhodes College, 2000 North Parkway, Memphis, TN 38112, USA

§Department of Biology, Yale University, New Haven, CT, USA

{varphi}Department of Medicine, Duke University, Durham, NC, USA

{circ}US Enviromnental Protection Agency, University of North Carolina, Chapel Hill, NC, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
P. Gunning, G. O'neill, and E. Hardeman
Tropomyosin-Based Regulation of the Actin Cytoskeleton in Time and Space
Physiol Rev, January 1, 2008; 88(1): 1 - 35.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M P Mullen, C W Smith, J G Patton, and B Nadal-Ginard
Alpha-tropomyosin mutually exclusive exon selection: competition between branchpoint/polypyrimidine tracts determines default exon choice.
Genes & Dev., April 1, 1991; 5(4): 642 - 655.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.