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Nucleic Acids Research, 1989, Vol. 17, No. 7 2819-2833
© 1989

MOLECULAR BIOLOGY

DNA topology of the ordered chromatin domain 5' to the human c-myc gene

S. Kumar and M. Leffak

Department of Biochemistry, Wright State University Dayton, OH 45435, USA

Received September 27, 1988. Revised March 2, 1989. Accepted March 2, 1989.

DNA restriction fragments located 5' to the human c-myc gene display anomalous electrophoretic mobility on polyacrylamide gels. Computer modeling of the c-myc flanking DNA suggests that the slow-moving DNA fragments spanning nucleotides –1690 to –1054 (relative to c-myc promoter P1) and –718 to –452 form large left handed superhelices or curved structures while the fast-moving DNA fragment spanning nucleotides –407 to + 78 has an unusually straight structure. These analyses also predict a periodic array of localized regions of bending through the superhelical domains. Microccocal nuclease digestion of isolated nuclei reveals that the slow-moving DNA fragments exist in an ordered chromatin structure stable to nuclease, whereas the digestion pattern of the fast-moving DNA fragment suggests a less ordered array of nucleosomes or a non-nucleosomal chromatin structure.


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