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Nucleic Acids Research, 1989, Vol. 17, No. 9 3491-3499
© 1989

ENZYMOLOGY

Identification of a novel site specific endonuclease produced by Mycoplasma fermentans: discovery while characterizing DNA binding proteins in T lymphocyte cell lines

Nancy F. Halden, Julie B. Wolf and Warren J. Leonard

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development NIH, Bethesda, MD 20892, USA

Received March 27, 1989. Revised April 3, 1989. Accepted April 3, 1989.

We have discovered a new restriction endonuclease, MfeI. in nuclear extracts from T cells contaminated with Mycoplasma fertmentans. This endonuclease was identified while studying proteins binding to the interleukin-2 receptor {alpha} chain gene promoter. MfeI cuts at the recognition sequence C'AATTG generating EcoRI compatible cohesive ends. Potential applications are discussed.


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