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Nucleic Acids Research, 1990, Vol. 18, No. 1 151-156
© 1990

MOLECULAR BIOLOGY

Cloning and sequence analysis of gyrA gene of Klebsiella pneumoniae

G.P. Dimri and H.K. Das*

Genetic Engineering Unit, Centre for Biotechnology, Jawaharlal Nehru University New Delhi 1 10 067, India

* To whom correspondence should be addressed

Received September 7, 1989. Accepted November 27, 1989.

The gene gyrA encoding the DNA gyrase A subunit of Klebsiella pneumoniae has been cloned in the plasmid pBR322. Bases of about 3.5 Kb DNA have been sequenced to locate the gyrA gene. An open reading frame of 2628 nucleotides coding for a 97 KD protein has been identified. Homology to the extent of about 85% was detected at the nucleotide level and about 90% at the amino acid level, when the sequences were compared with that of Escherichia coli gyrA. Some very interesting differences have, however, been found in the promoter region.


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