Nucleic Acids Research, 1990, Vol. 18, No. 1 23-34
© 1990
MOLECULAR BIOLOGY |
Analysis of the rat JE gene promoter identifies an AP-1 binding site essential for basal expression but not for TPA induction
Laboratory for Molecular Carcinogenesis, Sylvius Laboratories, University of Leiden PO Box 9503, 2300 RA Leiden, The Netherlands
*To whom correspondence should be addressed
Received November 8, 1989. Accepted December 1, 1989.
We have cloned the immediate-early serum-reponsive JE gene from the rat in order to study the regulation of this gene. We show that sequences of the JE promoter region confer serum-inducibility to a reporter gene. Analysis of the promoter in transient assays reveals that: i) the –141/–88 region is required for the response to the phorbol ester TPA, ii) the –70/–38 region is essential for basal activity. This latter region harbors the sequence TGACTCC, which resembles the consensus site for AP-1 binding, TGACTCA. DNA-protein binding assays indicate that the JE AP-1 site and the consensus AP-1 site have an overlapping but not identical binding spectrum for AP-1 proteins. Our data suggest that the inability of some AP-1 sites to respond to TPA is caused by subtle differences in affinity for AP-1 proteins.
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