Nucleic Acids Research, 1990, Vol. 18, No. 10 3027
© 1990
MOLECULAR BIOLOGY |
Identification of tissue specific nuclear proteins: DNA sequence and protein binding regions in the T cell receptor beta J-C intron
Division of Immunology, Department of Pathology, University of Pennsylvania Philadelphia, PA 19104 1Dana-Farber Cancer Institute and Department of Biological Chemistry, Harvard Medical School Boston, Massachusetts 02115, USA
*To whom correspondence should be addressed at University of Pennsylvania, Department of Pathology and Laboratory Medicine, John Morgan Building, Room 269, Philadelphia, PA 19104-6082, US
Received October 18, 1989. Accepted April 17, 1982.
We have determined the DNA sequences in the J2–C2 intron of the T cell receptor (TCR) beta gene and analyzed nuclear proteins binding to this region.Previously, we identified two tissue-specific DNase I hypersensitive regions, potential regulatory regions, In the J-C intron. The DNA sequence of the J2-C2 intron revealed that both DNase I hypersensitive regions have similar DNA sequences, suggesting that these regions are evolutionarily conserved. We have also dentified tissue-specific nuclear-protein binding regions downstream of the DNase hypersensitive regions. Although transcriptional enhancer activity was not observed in the hypersensitive regions or the adjacent protein binding regions In the J-C intron, our findings suggest that the TCR-beta J-C intron may contain some other type of regulatory element.