Skip Navigation

This Article
Right arrow Print PDF (734K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Eisenstein, M.
Right arrow Articles by Rabinovich, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eisenstein, M.
Right arrow Articles by Rabinovich, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 11 3185-3194
© 1990

CHEMISTRY

The structure and hydration of the A-DNA fragement d(GGGTACCC) at room temperature and low temperature

M. Eisenstein, F. Frolow, Z. Shakked and D. Rabinovich

Department of Structural Chemistry, The Weizmann Institute of Science Rehovot 76100, Israel

Received March 14, 1990. Accepted May 11, 1990.

The DNA fragment d(GGGTACCC) was crystallized as an A-DNA duplex in the hexagonal space group p61.The structure was analyzed at room temperature and low temperature (100K) at a esolution of 2.5 A. The helical conformations at the two temperatures are similar but the low-temperature structure is more economically hydrated than the room-temperature one. The structure of d(GGGTACCC) is compared to those of d(GGGTGCCC) and d(GGGCGCCC). This series of molecules, which consists of a mismatched duplex and its two Watson-Crick analogues, exhibits three conformational variants of the A-form of DNA, which are correlated with the specific intermolecular interactions observed In the various crystals. The largest differences in local conformation are displayed by the stacking geometries of the central pyrimldinepurfne and the flanking purine-pyrimidlne sites in each of the three duplexes. Stacking energy calculations performed on the crystal structures show that the mismatched duplex is destabilized with respect to each of the error-free duplexes, in accordance with helix-coil transition measurements.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Rozenberg, D. Rabinovich, F. Frolow, R. S. Hegde, and Z. Shakked
Structural code for DNA recognition revealed in crystal structures of papillomavirus E2-DNA targets
PNAS, December 22, 1998; 95(26): 15194 - 15199.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.