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Nucleic Acids Research, 1990, Vol. 18, No. 11 3255
© 1990

ENZYMOLOGY

Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites

Jianzhu Chen, Leonard A. Herzenberg and Leonore A. Herzenberg*

Department of Genetics, Stanford University Stanford, CA 94305, USA

*To Whom correspondence should be addressed

Received February 27, 1990. Accepted May 25, 1990.

Studies presented here demonstrate that heparin inhibits EcoR I endonuclease cleavage of DNA whereas related proteoglycans show no effect. The inhibition occurs at particular EcoR I sites that are near or overlap with palindromic sequences in the murine {lambda}5 and Lyt-2 genes. Endogenous heparin from peritoneal mast cells co-isolates with DNA and inhibits digestion of peritoneal cell DNA at the inhibitable sites. Digestion of spleen DNA is inhibited at the same sites when commercial heparin is added prior to digestion. In both cases, the inhibition is abolished by pre-treating the DNA with heparinase. Thus, potential artifacts in restriction fragment length analyses could occur with DNA isolated either from cells that are naturally rich In heparin or from cells to which heparin has been added, e.g., as an anticoagulant.


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