Skip Navigation

This Article
Right arrow Print PDF (6701K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Burke, C.
Right arrow Articles by Ackerman, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burke, C.
Right arrow Articles by Ackerman, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 12 3611-3620
© 1990

MOLECULAR BIOLOGY

Transcription factor IIA of wheat and human function similarly with plant and animal viral promoters

Christine Burke, Xu-Bo Yu+, Louis Marchitelli§, Elizabethc A. Davis and Steven Ackerman*

Biology Department, University of Massachusetts Boston, MA 02125, USA

*To whom correspondence should be addressed

Received January 22, 1990. Revised May 8, 1990. Accepted May 8, 1990.

Eucaryotic transcription initiation by RNA polymerase II involves protein: DNA interactions during the formation of a transcription complex. In addition to RNA polymerase II there are at least five other general transcription factors necessary for initiation with the adenovirus major late promoter. One of these, TFIIA, is involved in the earliest events during transcription complex assembly. We have purified TFIIA from wheat germ and characterized it in an in vitro transcription system. Wheat TFIIA is a single polypeptide of Mr ~35 kd which functionally replaces human (HeLa) TFIIA to form a wheat/HeLa transcription system. [This polypeptide can be eluted from a SDS-polyacrylamide gel, refolded to a native conformation, and will function as wheat TFIIA in the heterologous system.] The heterologous system requires a lower optimal incubation temperature than the HeLa system. Biochemical characterization, using the adenovirus major late promoter, indicates that transcription reaction parameters for both wheat and HeLa TFIIA are similar but the kinetics of transcription for both TFIIAs are somewhat dissimilar. A plant viral promoter, the cauliflower mosaic virus 35S promoter, accurately and efficiently directs in vitro transcription in both the wheat/HeLa and HeLa systems with identical transcription kinetics. We conclude that TFIIA function has been conserved during evolution.

+Present addresses: Immulogic Pharmaceutical Corp., One Kendall Square, Cambridge, MA 02139

§Present addresses: Exercise Physiology Division, USARIEM, Kansas St Natick, MA 01760, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
J. Ranish, W. Lane, and S Hahn
Isolation of two genes that encode subunits of the yeast transcription factor IIA
Science, February 28, 1992; 255(5048): 1127 - 1129.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.