Nucleic Acids Research, 1990, Vol. 18, No. 14 4123-4130
© 1990
GENOME STRUCTURE AND MAPPING |
Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites
Nuffield Department of Surgery, John Radcliffe Hospital Headington, Oxford 0X3 9DU 1Transplantation Biology Section, Clinical Research Centre Watford Road, Harrow, Middlesex HA1 3UJ, UK
Received April 26, 1990. Revised May 31, 1990. Accepted May 31, 1990.
Fifty sequences from the mouse genome database containing simple sequence repeats or microsatellites have been analysed for size variation using the polymerase chain reaction and gel electrophoresis. 88% of the sequences, most of which contain the dinucleotide repeat, CA/GT, showed size variations between different inbred strains of mice and the wild mouse, Mus spretus. 62% of sequences had 3 or more alleles. GA/CT and AT/TA -containing sequences were also variable. About half of these size variants were detectable by agarose gel electrophoresis. This simple approach is extremely useful in linkage and genome mapping studies and will facilitate construction of high resolution maps of both the mouse and human genomes.
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