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Nucleic Acids Research, 1990, Vol. 18, No. 15 4395-4400
© 1990

MOLECULAR BIOLOGY

Distortions induced in double-stranded oligonucleotides by the binding of cis- or trans-diammine-dichloroplatinum(ll) to the d(GTG) sequence

Marie-France Anin and Marc Leng

Centre de Biophysique Moléculaire CNRS, 45071 Orléans cedex 2, France

Received May 21, 1990. Revised July 9, 1990. Accepted July 9, 1990.

Conformational changes induced in double-stranded oligonucleotides by the binding of trans- or cis-diamminedichloro platinum(ll) to the d(GTG) sequence have been characterized by means of melting temperatures, electrophoretic migrations in nondenaturing polyacrylamide gels, reactivities with the artificial nuclease Phenanthroline-copper and with chemical probes. The cis-platinum adduct behaves more as a centre of directed bend than as a hinge joint, the induced bend angle being of the order of 25–30°. The double helix is locally denatured over 2 base pairs (corresponding to the platinated 5'G residue and the central T residue) and is distorted over 4 – 5 base pairs. The frans-platinum adduct behaves also more as a centre of directed bend than as a hinge joint, the induced bend angle being of the order of 60°. The double helix is locally denatured over 4 base pairs (corresponding to the immediately 5'T residue adjacent to the adduct and to the three base residues of the adduct).Both the cis- and trans-platinum adducts decrease the thermal stability of the double helix.


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