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Nucleic Acids Research, 1990, Vol. 18, No. 15 4417-4421
© 1990

GENOME STRUCTURE AND MAPPING

High speed DNA sequencing by capillary electrophoresis

John A. Luckey, Howard Drossman, Anthony J. Kostichka, David A. Mead, Jonathan D'Cunha, Tracy B. Norris and Lloyd M. Smith*

Department of Chemistry, University of Wisconsin Madison, Wl 53706, USA

*To whom correspondence should be addressed

Received May 17, 1990. Revised June 15, 1990. Accepted June 15, 1990.

A major challenge of the Human Genome Initiative is the development of a rapid, accurate, and efficient DNA sequencing technology. A major limitation of current technology is the relatively long time required to perform the gel electrophoretic separations of DNA fragments produced in the sequencing reactions. We demonstrate here that it is possible to increase the speed of sequence analysis by over an order of magnitude by performing the electrophoresis and detection in ultra thin capillary gels. An instrument which utilizes these high speed separations to simultaneously analyze many samples will constitute a second generation automated DNA sequencer suitable for large-scale sequence analysis.


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