Skip Navigation

This Article
Right arrow Print PDF (6066K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Prescott, C. D.
Right arrow Articles by Göringer, H.U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prescott, C. D.
Right arrow Articles by Göringer, H.U.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 18 5381-5386
© 1990

MOLECULAR BIOLOGY

A single mutation in 16S rRNA that affects mRNA binding and translation-termination

Catherine D. Prescott* and H.Ulrich Göringer

Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann, Ihnestrasse 73, 1000 Berlin 33, FRG

*To whom correspondence should be addressed

Received July 9, 1990. Accepted August 29, 1990.

A single base change in 16S rRNA (C726 to G) has previously been shown to have a dramatic effect on protein synthesis in E. coli(1). This paper more specifically details the effects of the mutation on mRNA binding and translation-termination. The in vitrotechnique of toeprinting (2) was used to demonstrate that 30S subunits containing the mutation 726G had an altered binding affinity for mRNA by comparison to the wild type. In addition, expression of the mutant ribosomes in vivo resulted in exclusive suppression of the UGA nonsense codon. This effect was supported by in vitrostudies that showed the mutant ribosomes to have an altered binding affinity for Release Factor-2.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.