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Nucleic Acids Research, 1990, Vol. 18, No. 18 5419-5423
© 1990

CHEMISTRY

Site specific functionalization of oligonucleotides for attaching two different reporter groups

Sudhir Agrawal* and Paul C. Zamecnik

Worcester Foundation for Experimental Biology Maple Avenue, Shrewsbury, MA 01545, USA

*To whom correspondence should be addressed

Received June 25, 1990. Revised August 14, 1990. Accepted August 14, 1990.

The synthesis of an oligonucleotide functionalized to attach two different reporter groups at specific internucleotide linkages is described. To Incorporate the amine specific reporter group the internucleotide linkage is modified to phosphoramidate (N-1-aminoalkyl) and for a thiol specific reporter group the internucleotide linkage is modified to a phosphorothioate diester. The synthetic cycle for introducing the modified internucleotide linkages at specific sites can be carried out using an automated DNA synthesizer. Combination of reporter groups have been attached successfully.


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Y. Aubert, S. Bourgerie, L. Meunier, R. Mayer, A.-C. Roche, M. Monsigny, N. T. Thuong, and U. Asseline
Optimized synthesis of phosphorothioate oligodeoxyribonucleotides substituted with a 5'-protected thiol function and a 3'-amino group
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