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Nucleic Acids Research, 1990, Vol. 18, No. 19 5673-5676
© 1990

MOLECULAR BIOLOGY

Rat DNA polymerase ß gene can join in excision repair of Escherichia coli

Takeo Ohnishi*, Shunsuke Yuba, Takayasu Date1, Hiroshi Utsumi2 and Akio Matsukage3

Department of Biology, Nara Medical University Kashihara, Nara 634 1Department of Biochemistry, Kanazawa Medical University Daigaku, Uchinada-cho, Kahoku-gun, Ishikawa-ken 902-02 2Radiation Biology Center, Kyoto University Yoshida, Konoe-cho, Sakyo-ku, Kyoto 606 3Laboratory of Cell Biology, Aichi Cancer Center Research Institute Chikusa-Ku, Nagoya 464, Japan

*To whom correspondence should be addressed

Received July 16, 1990. Revised September 3, 1990. Accepted September 3, 1990.

Though DNA polymerase I (poII) of Escherchia (E.) coli is understood to play a role In repair synthesis of excision repair, it Is still obscure whether DNA polymeraseß (poI/ß) plays a similar role in eukaryotic cells. To estimate the role of poIß in excision repair processes, we inserted the rat polß gene into several mutant E. coli defective in a diverse set of enzymatic activities of poII. UV resistance was seen only when the 5'-3'exonuclease (exo) activity of poII molecules remained. Therefore it is suggested that 5'-3'exo activity as well as polß activity are essential for repair synthesis of excision repair in eukaryotic cells.


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