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Nucleic Acids Research, 1990, Vol. 18, No. 19 5717-5721
© 1990

MOLECULAR BIOLOGY

The binding site for the liver-specific transcription factor Tf-LF1 and the TATA box of the human transferrin gene promoter are the only elements necessary to direct liver-specific transcription in vitro

Daniel Mendelzon, François Boissier and Mario M. Zakin

Laboratoire d'Expression des Génes Eucaryotes, Institut Pasteur 28 rue du Docteur Roux, 75724 Paris Cedex 15, France

*To whom correspondence should be addressed

Received July 3, 1990. Revised September 6, 1990. Accepted September 6, 1990.

We have studied the liver-specific transcriptional activity of the human transferrin gene promoter. Results of competition experiments, site-directed mutagenesis, and 5' deletion analysis have demonstrated that a TATA box and a binding site for the liver-specific protein Tf-LF1 are the only elements needed to direct hepatic-specific transcription in vitro. Thus, Tf-LF1 behaves as other previously described proteins, HNF-1, DBP and LF-A1, in that it Is sufficient to confer liver-specific transcriptional activity to a promoter in vitro. This results contrast with observations made in transient expression experiments, in which Tf-LF1 binding alone cannot direct hepatic-specific expression, and the binding of at least one more protein, similar to C/EBP, Is needed. Thus, as described for other hepatic genes, the number of elements necessary to confer tissue specificity is different in vivo and in vitro.


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