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Nucleic Acids Research, 1990, Vol. 18, No. 2 351-353
© 1990

CHEMISTRY

Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure

Saverio Alberti* and Mara Fornaro

lstituto di Ricerche Farmacologiche ‘Mario Negri’, Via Eritrea 62, 20157 Milan, Italy

* To whom correspondence should be addressed

Received November 20, 1989. Accepted December 4, 1989.

Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford (1,2) to include a treatment with guanidinium thiocyanate. The DNA obtained following the present protocol transfects eukaryotic cells with higher efficiency.


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