Nucleic Acids Research, 1990, Vol. 18, No. 21 6253-6260
© 1990
MOLECULAR BIOLOGY |
Binding sites in mammalian genes and viral gene regulatory regions recognized by methylated DNA-binding protein
Department of Biochemistry, Tulane Medical School New Orleans, LA 70112 1Southem Regional Research Center, US Department of Agriculture New Orleans, LA 70179, USA
*To whom correspondence should be addressed
Received July 31, 1990. Revised September 18, 1990. Accepted September 18, 1990.
Methylated DNA-binding protein (MDBP), a ubiquitous mammalian protein, recognizes a variety of related DNA sequences. Some of these sequences require methylation of their CpG dinucleotides for binding and others do not. We report that MDBP binds, in a DNA methylation-independent fashion, to two sites in the mouse polyomavirus enhancer, one in the enhancer of the human hepatitis B virus, and to one in the long terminal repeat of equine infectious anemia proviral DNA. We have also found a number of MDBP sites in human and rodent DNAs which bind much better to MDBP when they are methylated at CpG dinucleotides within the recognition site. These include sites at the beginning of the human genes for hypoxanthlne phosphorlbosyl transferase, HLA-A2, -A3, and -A25 antigens, and 
-galactosidase A. In the case of methylation-responsive MDBP sites, changes in their methylation status during differentiation or DNA replication could help drive development by modulating transcription.
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