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Nucleic Acids Research, 1990, Vol. 18, No. 21 6413-6417
© 1990

MOLECULAR BIOLOGY

A method for determining the relative effect of ligands on A-T and G-C base pairs in DNA: applications to metal ions, protons and two amino acids

S.G. Haroutiunian, E.B. Dalian, V.M. Aslanian, D.Yu. Lando1 and A.A. Akhrem1

Department of Molecular Physics and Biophysics, Yerevan State University USSR, Yerevan 375049, Mravian 1 1Institute of Bioorganic Chemistry of Academy of Science, Belorussian SSR, Minsk 220600, Zjodinskaya, 5, USSR

Received December 6, 1990. Revised October 3, 1990. Accepted October 3, 1990.

A new method is described for the study of specific interactions of low-molecular ligands with the base pairs of DNA. This method is based on the comparative analysis of melting temperature changes in DNAs of different GC-content in the presence of low molecular weight ligands.

In this paper, the method Is applied to Mn2+, NI2+, Co2+ ions, deprotonation, amino acids, ß-alanine and {gamma}-aminobutyrlc acid ({gamma}-ABA). Differences in Tm are affected not only by the changes of relative stability of AT- and GC-pairs, but also by other factors.

A theoretical analysis of the sequence specificity of low-molecular ligands on the base pairs in DNA molecules characterized by a high degree of sequence heterogeneity is also presented.


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