Nucleic Acids Research, 1990, Vol. 18, No. 22 6559-6564
© 1990
MOLECULAR BIOLOGY |
Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae
Institute of Cell and Molecular Biology, University of Edinburgh King's Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
*To whom correspondence should be addressed
Received August 7, 1990. Revised October 15, 1990. Accepted October 15, 1990.
PRP2 protein of Saccharomyces cerevisiae is required for the pre-mRNA splicing reaction but not for the early stages of spliceosome assembly. Using anti-PRP2 antibodies we demonstrate that PRP2 protein is associated with spliceosomes prior to, and throughout step 1 of the splicing reaction. Heat-inactivated prp2 protein, by contrast, does not seem to associate with spliceosomes. By elution of electrophoretically distinct spliceosomal complexes from non-denaturing gels we identify the specific complex with which PRP2 initially interacts in the pathway of spliceosome assembly.
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