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Nucleic Acids Research, 1990, Vol. 18, No. 23 6997-7002
© 1990

Articles

A simple and sensitive method for determining transcription initiation site: identification of two transcription initiation sites in rat group II phospholipase A2 gene

Osamu Ohara, Jun Ishizaki, Tohru Nakano, Hitoshi Arita and Hiroshi Teraoka

Shionogi Research Laboratories, Shionogi and Co. Ltd Fukushima-Ku, Osaka 553, Japan

Received August 3, 1990. Revised October 25, 1990. Accepted October 25, 1990.

We developed a simple and sensitive method for assigning transcriptional initiation sites, and applied it to characterize the transcriptional unit of rat group II phospholipase A2 (PLA2) gene. Our method involves the primer extension reaction followed by detection of its products by hybridization. Using this method, we were able to map two transcriptional initiation sites on the nucleotide sequence of the core promoter region of PLA2 gene with one-base resolution without any difficulties.


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