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Nucleic Acids Research, 1990, Vol. 18, No. 24 7229-7234
© 1990


Articles

Identification and purification of a Bombyx mori homologue of FTZ-F1

Hitoshi Ueda* and Susumu Hirose1

Genetic Stock Center, National Institute of Genetics Mishima 411, Japan 1DNA Research Center, National Institute of Genetics Mishima 411, Japan

*To whom correspondence should be addressed

Received October 11, 1990. Revised November 21, 1990. Accepted November 21, 1990.

Extracts from embryos and from posterior and middle silk glands of the silkworm, Bombyx mori contain a sequence specific DNA binding factor termed BmFTZ-F1. The factor binds to the recognition site of FTZ-F1, a positive regulator of the fushi tarazu gene in Drosophlla melanogaster. BmFTZ-F1 and FTZ-F1 share the same methylation interference patterns, the same chromatographic behaviors and similar protease digestion profiles. Anti-FTZ-F1 cross reacts with BmFTZ-F1. These results indicate that BmFTZ-F1 is a B.mori homologue of FTZ-F1. The mobility of the factor-DNA complex formed in the silk gland extract changes depending on the developmental stages. Purification of BmFTZF1 to an almost homogeneous state reveals that the factor is a 73 kd protein.


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