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Nucleic Acids Research, 1990, Vol. 18, No. 3 459-464
© 1990

GENOME STRUCTURE AND MAPPING

DNase I-defined chromatin configuration of the human CD3 gene cluster

A. Tunnacliffe

Basel Institute for Immunology Grenzacherstrasse 487, Postfach, CH-4005 Basel, Switzerland

Received November 10, 1989. Revised January 2, 1990. Accepted January 2, 1990.

The three CD3 genes on human chromosome 11q23 encode proteins ({gamma}, {delta} and {varepsilon}) which form part of the antigen receptor on T lymphocytes. All three genes are clustered within 50 kb and are activated approximately contemporaneously during the early stages of T cell ontogeny. In order to pinpoint potential regulatory sequences important for locus activation and tissue-specific gene expression, the chromatin structure of almost 90 kb of this region has been probed in five cell lines using the endonuclease pancreatic DNase I. A set of DNase I hypersensitive (HS) sites has been defined in T cell chromatin, five of which were strong and not found in non-I cells, with the exception of the erythroleukaemia cell line K562, in which three sites were weakly expressed, correlating with a low level of {delta} mRNA. The subset of five HS sites map close to the CD3 genes and lie in regions which contain elements of defined function: the {gamma} promoter; the {delta} promoter and its 3' enhancer; and the {varepsilon} promoter and its 3' enhancer. Since no further major T cell-restricted HS sites lie within the 90kb of the CD3 locus analysed, these five regions may contain all the sequences important for CD3 gene expression.


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