Skip Navigation

This Article
Right arrow Print PDF (4717K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (72)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Turmel, C.
Right arrow Articles by Noolandi, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Turmel, C.
Right arrow Articles by Noolandi, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 3 569-575
© 1990

MOLECULAR BIOLOGY

Molecular detrapping and band narrowing with high frequency modulation of pulsed field electrophoresis

Chantal Turmel1,2, Eric Brassard1,2, Gary W. Slater and Jaan Noolandi*

1Xerox Research Centre of Canada 2660 Speakman Drive, Mississauga, Ontario L5K 2L1, Canada 2Biotechnology Research Institute 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada

* To whom correspondence should be addressed

Received October 3, 1989. Revised December 11, 1989. Accepted December 11, 1989.

In high electric fields, megabase DNA fragments are found to be trapped, i.e. to enter or migrate in the gel only very slowly, if at all, leading to very broad electrophoretic bands and loss of separation. As a consequence, low electric fields are usually used to separate these molecules by pulsed field electrophoretic methods. We report here that high-frequency pulses eliminate the molecular trapping found in continuous fields. When high frequency pulses are used to modulate the longer pulses used in pulsed field electrophoresis, narrower bands result, and higher fields can be used. We suggest that this is due to effects that occur on the length scale of a single pore.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Gurrieri, S. B. Smith, and C. Bustamante
Trapping of megabase-sized DNA molecules during agarose gel electrophoresis
PNAS, January 19, 1999; 96(2): 453 - 458.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J.-L. Viovy and T. Duke
Solid Friction and Polymer Relaxation in Gel Electrophoresis
Science, April 1, 1994; 264(5155): 112 - 113.
[PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.