Nucleic Acids Research, 1990, Vol. 18, No. 5 1145-1152
© 1990
MOLECULAR BIOLOGY |
Cloning, characterization, and expression in Escherichia coli of the gene coding for the CpG DNA methylase from Spiroplasma sp. strain MQ1(M Sssl)
Department of Cellular Biochemistry Jerusalem, 91010 Israel 1Department of Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School Jerusalem, 91010 Israel 2New England Biolabs Inc. Beverly, MA 01915-9990, USA
Received December 12, 1989. Revised January 16, 1990. Accepted January 16, 1990.
We describe here the cloning, characterization and expression in E. coli of the gene coding for a DNA methylase from Spiropiasma sp. strain MQ1 (M Sssl). This enzyme methylates completely and exclusively CpG sequences (1). The Spiroplasma gene was transcribed In E. coli using its own promoter. Translation of the entire message required the use of an opal suppressor, suggesting that UGA triplets code for tryptophan in Spiroplasma. Sequence analysis of the gene revealed several UGA triplets, in a 1158 bp long open reading frame. The deduced amino acid sequence revealed in M•Sssl all common domains characteristic of bacterial cytosine DNA methylases. The putative sequence recognition domain of M•Sssl showed no obvious similarities with that of the mouse DNA methylase, in spite of their common sequence specificity. The cloned enzyme methylated exclusively CpG sequences both in vivo and in vitro. In contrast to the mammalian enzyme which is primarily a maintenance methylase, M•Sssl displayed de novo methylase activity, characteristic of prokaryotic cytosine DNA methylases.
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