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Nucleic Acids Research, 1990, Vol. 18, No. 5 1167-1173
© 1990

MOLECULAR BIOLOGY

Expression of adenovirus type 2 DNA polymerase in insect cells infected with a recombinant baculovirus

Christine J. Watson and Ronald T. Hay

Department of Biochemistry and Microbiology, University of St Andrews North Street, St Andrews, Fife KY16 9AL, UK

Received December 4, 1989. Revised February 9, 1990. Accepted February 9, 1990.

Sequences encoding adenovirus type 2 DNA polymerase were placed under control of the polyhedrin promoter and inserted into the baculovirus Autographa californica nuclear polyhedrosis virus by homologous recombination. Insect cells infected with the recombinant virus produced substantial amounts of the adenovirus type 2 DNA polymerase protein which was functional in both DNA polymerase and replication initiation reactions. Thus, the baculovirus expression system can provide active adenovirus type 2 DNA polymerase that is produced in quantities suitable for biochemical and structural analysis.


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