Skip Navigation

This Article
Right arrow Print PDF (3202K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Peek, R.
Right arrow Articles by Schoenmakers, J. G.G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peek, R.
Right arrow Articles by Schoenmakers, J. G.G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 5 1189-1197
© 1990

MOLECULAR BIOLOGY

Tissue- and species-specific promoter elements of rat {gamma}-crystallin genes

Ron Peek, Paul van der Logt, Nicolette H. Lubsen and John G.G. Schoenmakers*

Department of Molecular Biology, University of Nijmegen Toernooiveld, 6525 ED Nijmegen, The Netherlands

*To whom correspondence should be addressed

Received November 17, 1989. Revised February 13, 1990. Accepted February 13, 1990.

The 5' flanking regions of the six rat {gamma}-crystallin genes ({gamma}A-{gamma}F) are all capable of conferring lens-specific expression to the bacterial chioramphenicol acetyl transferase (CAT) reporter gene in either transdifferentiating chicken neural retina cells or mouse lens epithelial cells. Deletion mapping of the moat active {gamma}-crystallin promoter region, the {gamma}D region, showed that at least three elements are required for maximal expression in mouse lens epithelial cells: element(s) located between –200 and –106, a conserved CG rich region around position –75, and a CG stretch around –15. The region between –200 and –106 was dispensable in transdifferentiating chicken neural retina cells, which instead required the region between –106 and –78. The maximal activity of the {gamma}E and {gamma} promoters was also dependent upon the integrity of the conserved CG region located around –75. A synthetic oligonucleotide containing this sequence was capable of lens-specific enhancement of the activity of the tk promoter in transdifferentiating chicken neural retina cells but not in mouse lens epitheilal cells. Our results further show that this region may contain a silencer element, active in non-lens tissues, as well.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
A. Civil, S. T. van Genesen, and N. H. Lubsen
c-Maf, the {gamma}D-crystallin Maf-responsive element and growth factor regulation
Nucleic Acids Res., February 15, 2002; 30(4): 975 - 982.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Lengler, E. Krausz, S. Tomarev, A. Prescott, R. A. Quinlan, and J. Graw
Antagonistic action of Six3 and Prox1 at the {{gamma}}-crystallin promoter
Nucleic Acids Res., January 15, 2001; 29(2): 515 - 526.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. J. Klok, S. T. van Genesen, A. Civil, J. G. G. Schoenmakers, and N. H. Lubsen
Regulation of Expression within a Gene Family. THE CASE OF THE RAT gamma B- AND gamma D-CRYSTALLIN PROMOTERS
J. Biol. Chem., July 3, 1998; 273(27): 17206 - 17215.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.