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Nucleic Acids Research, 1990, Vol. 18, No. 6 1333-1338
© 1990

MOLECULAR BIOLOGY

Identification and sequence analysis of the ribosomal DNA promoter region of Crithidia fasciculata

Ernst J.M. Grondal, Raymond Evers and Albert W.C.A. Cornelissen*

Max-Planck-lnstitut für Biologie, Molecular Parasitology Unit Spemannstrasse 34, 7400 Tübingen, FRG

*To whom correspondence should be addressed

Received January 25, 1990. Accepted February 27, 1990.

We have identified the promoter region of the large ribosomal DNA repeat unit of Crithidia fasciculata by northern blotting and nuclear run-on analyses. These data show that transcription starts approximately 1 kb upstream of the 18S rRNA gene. S1 protection experiments and sequence analysis of this area resulted in a precise localization of the start site. We have been unable to Identify conserved sequence element(s) by a direct comparison of the crithidial RNA polymerase I promoter region and similar promoter regions of other eukaryotes; not even to the promoter region of the more closely related kinetoplastid species, Trypanosoma brucei. The absence of homology within the primary sequence of the promoter region, which is also found in other eukaryotes, might explain the observed species specificity of in vivo and in vitro rDNA transcription, since this resides in the interaction of initiation factor(s) and the core promoter domain.


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M. N. Schnare, J. C. Collings, D. F. Spencer, and M. W. Gray
The 28S-18S rDNA intergenic spacer from Crithidia fasciculata: repeated sequences, length heterogeneity, putative processing sites and potential interactions between U3 small nucleolar RNA and the ribosomal RNA precursor
Nucleic Acids Res., September 15, 2000; 28(18): 3452 - 3461.
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