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Nucleic Acids Research, 1990, Vol. 18, No. 6 1351-1359
© 1990

MOLECULAR BIOLOGY

Purification to homogeneity and partial amino acid sequence of a fragment which includes the methyl acceptor site of the human DNA repair protein for O6-methylguanine

G.N. Major*, E.J. Gardner1, A.F. Carne2 and P.D. Lawley

Alkylation Carcinogenesis Team, Chemical Carcinogenesis Section, Institute of Cancer Research Chester Beatty Laboratories, Fulham Road, London SW3 6JB, UK 1CRC Unit of Human Cancer Genetics, Department of Pathology, University of Cambridge Tennis Court Road, Cambridge CB2 1QP 2Department of Protein Chemistry, Celltech Ltd. Bath Road, Slough SL1 4EN, UK

*To whom correspondence should be addressed

Received January 19, 1990. Revised February 27, 1990. Accepted February 27, 1990.

DNA repair by O6-methyl transferase (O6-methylguanine-DNA methyl-transferase (O6-MT) is accomplished by removal by the enzyme of the methyl group from premutagenic O6-methylguanine-DNA, thereby restoring native guanine in DNA. The methyl group is transferred to an acceptor site cysteine thiol group in the enzyme, which causes the irreversible inactivation of O6-MT.

We detected a variety of different forms of the methylated, inactivated enzyme in crude extracts of human spleen of molecular weights higher and lower than the usually observed 21 – 24kDa for the human O6-MT. Several apparent fragments of the methylated form of the protein were purified to homogeneity following reaction of partially-purified extract enzyme with O6-[3H-CH3]methylguanine-DNA substrate. One of these fragments yielded amino acid sequence information spanning fifteen residues, which was identified as probably belonging to human methyltransferase by virtue of both its significant sequence homology to three procaryote forms of O6-MT encoded by the ada, ogt (both from E. coli) and dat (B. subtilis) genes, and sequence position of the radiolabelled methyl group which matched the position of the conserved procaryote methyl acceptor site cysteine residue. Statistical prediction of secondary structure indicated good homologies between the human fragment and corresponding regions of the constitutive form of O6-MT in procaryotes (ogt and dat gene products), but not with the inducible ada protein, indicating the possibility that we had obtained partial amino acid sequence for a non-inducible form of the human enzyme. The identity of the fragment sequence as belonging to human methyltransferase was more recently confirmed by comparison with cDNA-derived amino acid sequence from the cloned human O6-MT gene from HeLa cells (1). The two sequences compared well, with only three out of fifteen amino acids being different (and two of them by only one nucleotide in each codon).


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