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Nucleic Acids Research, 1990, Vol. 18, No. 6 1441-1446
© 1990

MOLECULAR BIOLOGY

Specific transcription of cloned Methanobacterium thermoautotrophicum transcription units by homologous RNA polymerase in vitro

Sigurd Knaub and Albrecht Klein

Molecular Genetics, Department of Biology, Philipps University PO Box 1929, D-3550 Marburg, FAG

Received December 13, 1989. Revised February 16, 1990. Accepted February 16, 1990.

Specific in vitro transcription by partially purified RNA polymerase from Methanobacterium thermoautotrophicum of DNA sequences cloned in between the promoter and terminator regions of the methyl CoM reductase transcription unit of the same organism is described. The 5'-terminus of the product has been mapped. Deletion analyses of the promoter region show the limits of the sequences essential for the promoter function.


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T. J. Darcy, W. Hausner, D. E. Awery, A. M. Edwards, M. Thomm, and J. N. Reeve
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