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Nucleic Acids Research, 1990, Vol. 18, No. 6 1513-1516
© 1990

MOLECULAR BIOLOGY

Retropseudogenes constitute the major part of the human elongation factor 1{alpha} gene family

Hans O. Madsen, Knud Poulsen1, Otto Dahl2, Brian F.C. Clark3 and J.Peter Hjorth

Department of Molecular Biology and Plant Physiology, Aarhus University DK-8000 Aarhus C 1Department of Oral Biology, Royal Dental College, Vennelyst Boulevard DK-8000 Aarhus C 2Department of General and Organic Chemistry, Copenhagen University DK-2100 Copenhagen Ø 3Department of Biostructural Chemistry, Department of Chemistry, Aarhus University DK-8000 Aarhus C, Denmark

Received November 29, 1989. Revised February 19, 1990. Accepted February 19, 1990.

The elongation factor la (EF-1{alpha}) is a protein which promotes the GTP-dependent binding of aminoacyl tRNA to ribosomes in the protein synthesis process. A human gene coding for EF-1{alpha} has previously been cloned and sequenced along with a pseudo-gene [1]. Here, we have further analyzed the family of human EF-1{alpha} genes. Using an EF-1{alpha} cDNA as probe twelve genomic EF-1{alpha}-like clones were isolated and analyzed. Four of these were sequenced and found to contain EF-1{alpha} retropseudogenes. A Southern blot analysis indicated that the remaining eight clones also contained retropseudogenes. Genomic Southern blot analysis revealed at least twenty loci in the human genome with sequence homology to the EF-1{alpha} cDNA. Besides the already described active gene only one potentially active locus was found. The others appeared to be retropseudogenes. EF-1{alpha} retropseudogenes were also found to be abundant in the mammalian species mouse and pig, while the chicken contained only one presumably active EF-1{alpha} gene.


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