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Nucleic Acids Research, 1990, Vol. 18, No. 7 1771-1781
© 1990

MOLECULAR BIOLOGY

Alternative DNA-protein interactions in variable-length internucleosomal regions associated with Drosophila Adh distal promoter expression

Amy Ewel, Jeffrey R. Jackson and Cheeptip Benyajati*

Department of Biology, University of Rochester Rochester, NY 14627, USA

* To whom correspondence should be addressed

Received December 12, 1989. Revised March 1, 1990. Accepted March 1, 1990.

Chromatin at the Drosophila Adh distal promoter displays an ordered but different conformation in different cell types as detected by a modified exonuclease protection assay and accessibility to endonucleases. In cells not transcribing Adh (ADH–) sequences between –40 to +30 of the distal RNA initiation she exist as a DNA linker between positioned nucleosomes, and appear to interact with a specific DNA-binding protein. in contrast, a longer linker DNA, from –140 to +30, is bound in a multi-protein transcription initiation complex in cells that specifically transcribe the distal (adult) ADH RNA (ADH+A). These DNA-protein interactions can account for a localized open chromatin structure at the distal promoter in ADH+A cells. The observed mutually exclusive patterns of DNA-protein interactions in the linkers of different ADH cell types between –40 to +30 suggest a model for organizing alternative chromatin structure associated with gene regulation. Two DNA binding proteins, one being a TATA box binding factor, compete for overlapping sites to allow either assembly of a transcription initiation complex and transcription, or positioning of nucleosomes for stable repression.


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