Skip Navigation

This Article
Right arrow Print PDF (7041K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Zhang, L.
Right arrow Articles by Gralla, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, L.
Right arrow Articles by Gralla, J. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 7 1797-1803
© 1990

MOLECULAR BIOLOGY

In situ nucleoprotein structure involving origin-proximal SV40 DNA control elements

Li Zhang and Jay D. Gralla*

Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California 405 Hilgard Avenue, Los Angeles, CA 90024-1 569, USA

* To whom correspondence should be addressed

Received December 4, 1989. Revised February 25, 1990. Accepted February 25, 1990.

Nucleoprotein structures at the SV40 GGbox and adjacent AT-rich region have been probed by nucleases in permeabiilzed cells at nucleotide level resolution. The patterns of nuclease protection and hypersensitivity in these permeabilized cells that allow initiation of RNA and DNA synthesis are quite different from those observed in isolated nuclei that are inactive. Whereas simple DNA protection by factors is found in nuclei, the pattern in permeabilized cells includes very strong nuclease hypersensitive sites. Their arrangement suggests that the region exists as a higher order nucleoprotein complex in vivo, which is disturbed during the preparation of nuclei. The pattem is also found to be disturbed in permeabilized cells when T-antigen is inactivated by temperature-sensitive mutation. Since T-antigen origin binding sites and the GC-box region have been shown previously to interact functionally, the existence of a higher order structure involving both components provides a likely physical basis for the functional interaction of separate control elements.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. Kim, S. H. Yoshimura, K. Hizume, R. L. Ohniwa, A. Ishihama, and K. Takeyasu
Fundamental structural units of the Escherichia coli nucleoid revealed by atomic force microscopy
Nucleic Acids Res., April 1, 2004; 32(6): 1982 - 1992.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Pilartz and H. Jeske
Mapping of Abutilon Mosaic Geminivirus Minichromosomes
J. Virol., October 15, 2003; 77(20): 10808 - 10818.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Soldaini, M. Pla, F. Beermann, E. Espel, P. Corthésy, S. Barangé, G. A. Waanders, H. R. MacDonald, and M. Nabholz
Mouse Interleukin-2 Receptor [IMAGE] Gene Expression
J. Biol. Chem., May 5, 1995; 270(18): 10733 - 10742.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
G P Pfeifer
Analysis of chromatin structure by ligation-mediated PCR.
Genome Res., November 1, 1992; 2(2): 107 - 111.
[PDF]

Home page
 Genes Dev.Home page
G P Pfeifer and A D Riggs
Chromatin differences between active and inactive X chromosomes revealed by genomic footprinting of permeabilized cells using DNase I and ligation-mediated PCR.
Genes & Dev., June 1, 1991; 5(6): 1102 - 1113.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.