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Nucleic Acids Research, 1990, Vol. 18, No. 9 2653-2660
© 1990

GENOME STRUCTURE AND MAPPING

Ordering of cosmid clones covering the Herpes Simplex virus type I (HSV-I) genome: a test case for fingerprinting by hybridisation

A.G. Carig+, D. Nizetic, J.D. Hoheisel, G. Zehetner and H. Lehrach

Genome Analysis Laboratory, Imperial Cancer Research Fund Laboratory PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK

Received January 25, 1990. Accepted March 27, 1990.

To allow the efficient construction of ordered clone libraries, we have been investigating the use of ‘oligonucleotide fingerprinting’ as an approach to identify overlapping clones, and ultimately restore the linear order of the clone set. To test the effectiveness of the procedure, we have constructed a cosmid library from the genome of the human DNA virus HSV-I and used hybridisation to multiple oligonucleotldes selected from the nucleotide sequence to reconstruct the order of clones and oligonucleotides on the genome.

+Present address Molecular parasitology Group, Institute of Molecular Medicine, John Radeliffe Hospital, Headington, Oxford OX3 9DU, UK


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