Skip Navigation

This Article
Right arrow Print PDF (5405K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Maèno, M.
Right arrow Articles by Shikata, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maèno, M.
Right arrow Articles by Shikata, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 9 2685-2689
© 1990

Molecular Biology

A cDNA clone closely associated with non-A, non-B hepatitits

Mitsugo Maèno*, Kazuyoshi Kaminaka, Hiroyuki Sugimoto, Mariko Esumi, Nakanobu Hayshi, Kohei Komatsu, Kenji Abe1, Sadayoshi Sekiguchi2, Michitami Yano3, Kyosuke Mizuno4 and Toshio Shikata

Department of Pathology, Nihon University School of Medicine 30-1 Oyaguchikami-machi, Itabashiku, Tokyo 173 1Department of Pathology, National Institute of Healty Tokyo 2Hokkaido Red Cross Blood Center Sapporo 3Institute for Clinical Research, Nagasaki Chuo National Hospital Nagasaki 4Chemo-sero-therapeutic Research Institute Kumamoto, Japan

*To whom correspondence should be addressed

Received January 16, 1990. Accepted March 28, 1990.

A lambda gtl1 cDNA library was constructed from RNA purified from hepatitis B viral surface antigen-negative human plasma with high alanine aminotransferase activity. A cDNA clone, designated s C8-2, was isolated by immunoscreening with mixed sera from non-A, non-B hepatitis (NANBH) carrier and convalescent chimpanzees. The recombinant protein produced by C8-2 reacted specifically with sera of patients in the chronic phase of NANBH. The sequence of C8-2, 269 bp, did not hybridized with any human or chimpanzee genomic DNA, and had no homology with those of primates and viruses. The existence of this sequence in RNA of possibly infectious plasma was shown by RNA blot hybridization and by Southern blot analysis of products amplified by the polymerase chain reaction. These results strongly suggest that C8-2 is derived from the agent of this viral hepatitis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.