Nucleic Acids Research, 1991, Vol. 19, No. 10 2739-2746
© 1991
GENOME STRUCTURE AND MAPPING |
Characterizations of the human parainfluenza type 2 virus gene encoding the L protein and the intergenic sequences
1Department of Microbiology, Mie University School of Medicine 2-174 Edobashi, Tsu-Shi, Mie Prefecture 514 2Fujikura Research Center, Fujikura Kasei Co. Ltd 3-20-7 Hasune, Itabashi, Tokyo 174, Japan
* To whom correspondence should be addressed
Received December 31, 1990. Revised April 17, 1991. Accepted April 17, 1991.
We cloned and determined the nucleotide sequences of cDNAs against genomlc RNA encoding the L protein of human parainfluenza type 2 virus (PIV-2). The L gene is 6904 nucleotldes long including the intergenic region at the HN-L junction and putative negative strand leader RNA, almost all of which is complementary to the positive strand leader RNA of PIV-2. The deduced L protein contains 2262 amino acids with a calculated molecular weight of 256,366. The L protein of PIV-2 shows 39.9, 28.9, 27.8 and 28.3% homologies with Newcastle disease virus (NDV), Sendai virus (SV), parainfluenza type 3 virus (PIV-3) and measles virus(MV), respectively. Although sequence data on other components of transcriptive complex, NP and P, suggested a closer relationship between PIV-2 and MV, as concerns the L protein, MV Is closely related to another group as SV and PIV-3. From analysis of thealignment of the five L proteins, six blocks composed of conserved amino acids were found in the L proteins.The L protein of PIV-2 was detected in purified virions and virus-Infected cells using antlserum directed against an oligopeptlde corresponding to the amino terminal region. Primer extension analyses showed that the intergenic regions at the NP-P, P-M, M-F, F-HN and HN-L junctions are 4, 45, 28, 8 and 42 nucleotides long, respectively, indicating that the intergenic regions exhibit no conservation of length and sequence. Furthermore, the starting and ending sequences of paramyxoviruses were summarized.
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