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Nucleic Acids Research, 1991, Vol. 19, No. 10 2761-2765
© 1991

MOLECULAR BIOLOGY

The promoter of the endo A cytokeratin gene is activated by a 3' downstream enhancer

Yoshihiro Takemoto+, Yasuo Fujimura, Midori Matsumoto§, Yoshitaka Tamai, Takashi Morita, Aizo Matsushiro and Masami Nozaki*

Department of Microbial Genetics, Research Institute for Microbial Diseases, Osaka University 3-1, Yamadaoka, Suita, Osaka 565, Japan

* To whom correspondence should be addressed

Received December 11, 1990. Revised April 17, 1991. Accepted April 17, 1991.

Mouse cytokeratin EndoA is an intermediate filament subunit of the type II cytokeratin class which initiates expression in trophectoderm cells of blastocyst during embryogenesis. To identify the regulatory elements of the endo A gene, we constructed a series of CAT expression vectors and transfected them into PYS-2 cells. We found an enhancer element locating 1 kb downstream from the endo A gene which acts on both the endo A and SV40 promoters. This enhancer consists of six direct repeated sequences with homology to the PEA3 motif in polyoma virus {alpha} enhancer core. In undifferentiated F9 embryonal carcinoma cells, expression of the construct containing the enhancer was not detected. These results indicate that one of the regulatory mechanisms of endo A gene expression is the 3' downstream enhancer.

+ Present address: Tsukuba Life Science Center, The Institute of Physical and Chemical Research, 3-1-1, Koyadai, Tsukuba, Ibaraki 305

+ Present address: Department of Life Science, Faculty of Life Science and Technology, Tokyo Institute of Technology, Meguroku, Tokyo 152, Japan


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