Skip Navigation

This Article
Right arrow Print PDF (933K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (78)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lamm, G. M.
Right arrow Articles by Lamond, A. I
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lamm, G. M.
Right arrow Articles by Lamond, A. I
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1991, Vol. 19, No. 12 3193-3198
© 1991

MOLECULAR BIOLOGY

Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts

Gábor M. Lamm, Benjamin J. Blencowe, Brian S. Sparoat, Adolfo M. Iribarren, Ursula Ryder and Angus I Lamond*

EMBL Meyerhofstrasse 1, Posifach 102209, D6900 Heidelberg 1, FRG

* To whom correspondence should be addressed

Received April 29, 1991. Revised May 29, 1991. Accepted May 29, 1991.

RNA duplexes containing the modified base 2-amino-adenine in place of adenine are stabilized through the formation of three hydrogen bonds in 2-amino A· U base pairs. Antisense 2'-O-alkyloligorlbonucleotide probes incorporating 2-aminoadenosine are thus able to efficiently affinity select RNP particles which are otherwise inaccessible. This has allowed the efficient and specific depletion of U5 snRNP from HeLa cell nuclear splicing extracts. U5 snRNP is shown to be essential for spliceosome assembly and for both steps of pre-mRNA splicing. The absence of U5 snRNP prevents the stabte association of U4/U6 but not U1 and U2 snRNPs with pre-mRNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. He and F. Seela
Propynyl groups in duplex DNA: stability of base pairs incorporating 7-substituted 8-aza-7-deazapurines or 5-substituted pyrimidines
Nucleic Acids Res., December 15, 2002; 30(24): 5485 - 5496.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Gonzalez-Santos, A. Wang, J. Jones, C. Ushida, J. Liu, and J. Hu
Central Region of the Human Splicing Factor Hprp3p Interacts with Hprp4p
J. Biol. Chem., June 21, 2002; 277(26): 23764 - 23772.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Seela and G. Becher
Pyrazolo[3,4-d]pyrimidine nucleic acids: adjustment of dA-dT to dG-dC base pair stability
Nucleic Acids Res., May 15, 2001; 29(10): 2069 - 2078.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S Michaud and R Reed
A functional association between the 5' and 3' splice site is established in the earliest prespliceosome complex (E) in mammals.
Genes & Dev., June 1, 1993; 7(6): 1008 - 1020.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
B. Blencowe, M Carmo-Fonseca, S. Behrens, R Luhrmann, and A. Lamond
Interaction of the human autoantigen p150 with splicing snRNPs
J. Cell Sci., January 7, 1993; 105(3): 685 - 697.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
J R Wyatt, E J Sontheimer, and J A Steitz
Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing.
Genes & Dev., December 1, 1992; 6(12b): 2542 - 2553.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
U Utans, S E Behrens, R Luhrmann, R Kole, and A Kramer
A splicing factor that is inactivated during in vivo heat shock is functionally equivalent to the [U4/U6.U5] triple snRNP-specific proteins.
Genes & Dev., April 1, 1992; 6(4): 631 - 641.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.