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Nucleic Acids Research, 1991, Vol. 19, No. 6 1189-1195
© 1991

GENOME STRUCTURE AND MAPPING

Construction of an ordered clone bank and systematic analysis of the whole transcripts of chromosome VI of Saccharomyces cerevisiae

Akikazu Yoshikawa and Katsumi Isono*,1

Graduate School of Science and Technology, Faculty of Science, Kobe University Rokkodai, Kobe 657, Japan 1Department of Biology, Faculty of Science, Kobe University Rokkodai, Kobe 657, Japan

* To whom correspondence should be addressed

Received January 24, 1991. Revised February 27, 1991. Accepted February 27, 1991.

By comparing sequences of restriction enzyme cleavage sites and their distance data, we sorted 384 {lambda} phage clones containing segments of chromosome VI of S. cerevisiae and constructed an ordered clone bank for this chromosome. The physical length of this bank is 269.7 kb. The bank contains the entire chromosome including the left telomere, but it is not certain whether it contains the right telomere as well. To estimate the number of genes present on this chromosome, we performed a series of Northern hybridization experiments using 157 restriction enzyme fragments prepared from the bank as hybridization probes and total poly(A)+ RNA from vegetatlvely growing cells. Thus, 97 distinct transcripts were identified. The relative abundance levels of individual transcripts were measured by comparing their band intensity with that of the RPO41 transcript. It was found that the transcripts from the genes located in the telomeric and centromeric regions are less abundant as compared to those from the genes in the central regions of both arms.


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