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Nucleic Acids Research, 1975, Vol. 2, No. 10 1805-1810
© 1975

Articles

Thermal denaturation of B. subtilis DNA in H2O and D2O observed by electron microscopy

Vincenzo Izzo, Sandro L. Fornili and Lorenzo Cordone

Istituto di Fisica dell Universita and Gruppi Nazionali Struttura della Materia of the Consiglio Nazionale delle Ricerche Via Archirafi 36, 90123 Palermo, Italy

Received August 11, 1975. In the present work we studied the initial part of thermal denaturation curves of B. subtilis DNA in normal and heavy water, observing, by electron microscopy, the "opening" of DNA as a function of temperature. The results support the hypothesis that D2O plays a stabilizing role on strands separation during thermal denaturation of DNA.


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