Skip Navigation

This Article
Right arrow Print PDF (2075K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Edens, L.
Right arrow Articles by Schoenmakers, J. G.G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Edens, L.
Right arrow Articles by Schoenmakers, J. G.G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1975, Vol. 2, No. 10 1811-1820
© 1975

Articles

Physical mapping of the central terminator for transcription on the bacteriophage M13 genome

Luppo Edens, Ruud N.H. Konings and John G.G. Schoenmakers*

Laboratory of Molecular Biology, University of Nijmegen Nijmegen, The Netherlands

*Reprint requests to: Dr. John G.G.Schoenmakers, Laboratory of Molecular Biology, University of Nijmegen, Toernooiveld, Nijmegen, The Netherlands

Received August 13, 1975. With the aid of in vitro transcription and translation studies it has been demonstrated that termination of transcription on bacteriophage M13 replicative form DHA occurs at a unique site which is located immediately distal to the 3'-end of gene VIII, the gene which codes for the major capsid protein. The position of this site has been mapped accurately on the enzyme cleavage maps by transcription of restriction fragments of M13 RF DNA. The central termination site was found to be located in restriction fragment Hap-B2 at 450 nucleotides from the 5'-end of its viral strand (0.77 fractional length clockwise from the unique Hind II enzyme cleavage site).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.