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Nucleic Acids Research, 1975, Vol. 2, No. 12 2237-2250
© 1975

Articles

Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns

Don Eilat, Ehud Ziv and Yehuda Lapidot

Department of Biological Chemistry, The Hebrew University of Jerusalem Jerusalem, Israel

Received October 7, 1975. The o-nitrophenylsulfenyl group, an amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.


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