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Nucleic Acids Research, 1975, Vol. 2, No. 2 165-178
© 1975

Articles

Absence of mRNA for casein in free polysomes of lactating ewe mammary gland

L.M. Houdebine and P. Gaye

Laboratoire de Physiologie de la Lactation, Institut National de la Recherche Agronomique CNRZ, 78350-Jouy-en-Josas, France

Received December 19, 1974. Total polysomal RNA or poly A- containing RNA extracted from free polysomes of lactating ewe mammary gland was unable to direct a significant synthesis of {alpha}s-and ß-casein. Poly acrylamide gel electrophoresis of poly A- containing RNA revealed that the two major peaks of mRNA isolated from membrane-bound polysomes were not observed in mRNA isolated from free polysomes. Purified 125I anti-{alpha}s-casein could bind significantly to membrane-bound polysomes only. These data suggest that casein synthesis occurs almost exclusively on membrane-bound polysomes and that this specificity may be attributed to the presence of casein mRNAs on these polysomes and their absence from free polysomes.


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