Skip Navigation

This Article
Right arrow Print PDF (761K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (74)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lis, J. T.
Right arrow Articles by Schleif, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lis, J. T.
Right arrow Articles by Schleif, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1975, Vol. 2, No. 3 383-390
© 1975

Articles

Size fractionation of double-stranded DNA by precipitation with polyethylene glycol

John T. Lis and Robert Schleif

Brandeis University Waltham, Mass. 02154, USA

Received January 24, 1975. We show that DNA molecules of differing molecular mass are separable by selective precipitation with polyethylene glycol (PEG{dagger}). Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA. Double-stranded DNA can be fractionated at least in the range of 3 x 107 to 1 x 105 daltons. The effects of PEG concentration, sodium chloride concentration, DNA concentration, pH, divalent ions, precipitation time, and centrifugal force have been determined. These studies show PEG precipitation offers a size fractionation method for DNA which is convenient, of high capacity, and applicable over a wide range of conditions. However, resolution is not high and separation of two species approaches 100% only if they differ in molecular mass by at least a factor of two.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Am. J. Bot.Home page
S.-T. Kim and M. J. Donoghue
Incongruence between cpDNA and nrITS trees indicates extensive hybridization within Eupersicaria (Polygonaceae)
Am. J. Botany, September 1, 2008; 95(9): 1122 - 1135.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
Y.-C. Wang, T.-Y. Jian, L.-J. Tarn, Y.-W. Hung, H.-Y. Chao, D.-D. Ji, and H.-W. Liu
Development of a Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay and Its Applications in Field Surveillance of Rodent Mice for Presence of Immunoglobulin G against Orientia tsutsugamushi
Clin. Vaccine Immunol., May 1, 2003; 10(3): 451 - 458.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. C. Enright, N. P. J. Day, C. E. Davies, S. J. Peacock, and B. G. Spratt
Multilocus Sequence Typing for Characterization of Methicillin-Resistant and Methicillin-Susceptible Clones of Staphylococcus aureus
J. Clin. Microbiol., March 1, 2000; 38(3): 1008 - 1015.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
J P Jacques and M M Susskind
Pseudo-templated transcription by Escherichia coli RNA polymerase at a mutant promoter.
Genes & Dev., October 1, 1990; 4(10): 1801 - 1810.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
S M Liao, T H Wu, C H Chiang, M M Susskind, and W R McClure
Control of gene expression in bacteriophage P22 by a small antisense RNA. I. Characterization in vitro of the Psar promoter and the sar RNA transcript.
Genes & Dev., April 1, 1987; 1(2): 197 - 203.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.