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Nucleic Acids Research, 1975, Vol. 2, No. 4 603-611
© 1975

Articles

A rapid assay technique for RNA ribose methylases

Ingvar Svensson and Vinod J. Patel

Institute of Biochemistry, Biomedical Center, University ot Uppsala Box 576, S-751 23 Uppsala, Sweden

Received March 10, 1975. A rapid technique for quantitative separation of ribose-methylated nucleosides from base-methylated and non-methylated nucleosides by chromatography on DEAE-cellulose paper in the presence of borate is described. The method has been used as an assay for tRNA ribose r.ethylases from yeast, using undermethylated Escherichia coli tRNA as substrate. The main product formed with a partly purified yeast enzyme was characterized as 2'-O-methylcytidine.


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