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Nucleic Acids Research, 1975, Vol. 2, No. 5 635-646
© 1975

Articles

Enzymic in vitro repair and chemical nature of DNA chain breaks induced by incorporated phosphorus-32p decay

I. Fodor, N.I. Matvienko, K.M. Zlotnikov, V.I. Tanyashin, A.S. Solonin and A.A. Bayev

Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences Pushchino, 142292, USSR

Received January 30, 1975. In vitro repair of single strand breaks in T4 and {lambda} phage DNA caused by 32p decay was studied. Zone centrifugation procedure showed that polynucleotide kinasc, ligase enzyme system failed to repair 32P-damages. It was found that damaged DNA contained gaps and could be rcpaired by DNA-polymerase I, polynucleotide ligase treatment.


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