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Nucleic Acids Research, 1975, Vol. 2, No. 5 745-756
© 1975

Articles

A new assay of deoxyribonucleases using as a substrate radioactively labeled DNA bound either directly or through anti-DNA antibodies to plastic depression plates

Hanoch Slor

Department of Human Genetics, Sackler School of Medicine, Tel Aviv University Tel Aviv, Israel

Received April 4, 1975. A simple and accurate method for the detection of Deoxyribonucleases is described. The DNA substrate (3H-labeled) is bound to DNA-binding sites in the "wells" of plastic depression plates either directly of via anti-DNA antibodies. Following incubation with the appropriate enzyme, the radioactivity released from the wells or left bound to the wells is determined. The method is suitable for enzymes which attack native DNA, single stranded DNA and modified DNA (e.g., u.v.-irradiated) as substrates.


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