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Nucleic Acids Research, 1975, Vol. 2, No. 6 951-960
© 1975

Articles

The modified nucleosides of tRNAs. II. Synthesis of 2'-O-methylcytidylyl (3'-5') cytidine

W.T. Markiewicz and M. Wiewiórowski

Institute of Organic Chemistry, Polish Academy of Sciences ul.Noskowskiego 12/14, 61-704 Poznan, Poland

Received April 28, 1975. The synthesis of 2'-O-methylcytidylyl (3'-5')cytidine by the triester method using as protecting groups, 2,2,2 -trichloroethyl for phosphate hydroxyl group, pchlorophenoxyacetyl for 5-hydroxyl group, methoxymethylidene for 2',3'-cis-diol system, and benzoyl for the exo-amino group of cytidine is presented. The obtained product was characterised by UV, electrophoresis, chromatography and an enzymatic digestion.


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