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Nucleic Acids Research, 1975, Vol. 2, No. 7 1203-1212
© 1975

Articles

Hybridization of pigeon globin messenger RNA with complementary DNA synthesized in vitro by reverse transcription: influence of the homopolymeric regions

K.G. Gasaryan*, V.Z. Tarantul*, Yu.N. Baranov*, L.Yu. Frolova** and L.L. Kisselev**

*Kurchatov's Institute of Atomic Energy Moscow 123098 **Institute of Molecular Biology, Academy of Sciences of the USSR Moscow 117312, USSR

Accepted June 3, 1975.

The kinetics of hybridization of pigeon globin messenger RNA with complementary cDNA synthesized by means of AMV reverse transcriptase is complex. Addition of poly A or poly U in excess to the reaction mixture normalized the kinetics. It is concluded that association of the complementary homopolymeric regions of mRNA and cDNA accelerates the complex formation between heteropolymeric sequences in a fraction of the molecules.


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