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Nucleic Acids Research, 1975, Vol. 2, No. 8 1329-1344
© 1975

Articles

m7G5' ppp5' GmpCpUp at the 5' terminus of reovirus messenger RNA

M. Faust*, K.E.M. Hastings and S. Milward+

Department of Biochemistry, McGill University Montreal, Canada

Received June 18, 1975.

In the presence of S-adenosyl methionine the 5' terminal guanosine residue of in vitro synthesized reovirus mRNA becomes methylated at the 2'-OH position. In addition, 7-methyl guanylic acid is condensed covalently at the 5' terminus resulting in the formation of a 5' to 5' triphosphate bridge. Analysis of the 5' terminal sequence of methylated reovirus mRNA revealed that it has thestructure m7G5'ppp5' GmpCpUp.

*Holder of a Medical Research Studentship (Ottawa).

+Medical Research Council Scholar (Ottawa).


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